Superstimulatory Influenza Virus and Highly Organized BCR-Ligands Act Synergistically on B Cell Activation
Identifieur interne : 000284 ( France/Analysis ); précédent : 000283; suivant : 000285Superstimulatory Influenza Virus and Highly Organized BCR-Ligands Act Synergistically on B Cell Activation
Auteurs : Ortwin Rott [France] ; James J. Mond [États-Unis] ; Evelyne Cash [France]Source :
- Immunobiology [ 0171-2985 ] ; 1996.
English descriptors
- Teeft :
- Activation, Adsorption values, Antigen organization, Cell activation, Cell activators, Cell encounter, Cell proliferation, Cell proliferative responses, Cell responses, Cell responsiveness, Cell stimulation, Cell subpopulation, Cell subpopulations, Cells cells, Cholera toxin, Clear differences, Cochin hospital, Culture medium, Culture period, Different amounts, Different concentrations, Different forms, First example, Flow cytometry, High amounts, High concentrations, High rate, Immunoglobulin, Immunoglobulin synthesis, Immunol, Independent experiments, Influenza, Influenza virus, Influenza virus glycoprotein hemagglutinin, Influenza virus hemagglutinin, Influenza viruses, Inhibitory, Inhibitory effects, Lymphocyte, Lymphocyte activation, Maximal responses, Measurable production, Molecular strategy, Mond, Murine, Neonatal mice, Obligatory role, Other hand, Pathway, Phorbol esters, Phosphodiesterase inhibitor pentoxifylline, Proliferative, Proliferative responses, Reagent, Receptor, Respective stimulus, Responder cells, Rott, Saline solution, Saturating concentrations, Side scatter parameters, Significant proliferative responses, Similar observations, Small amounts, Soluble, Soluble antibodies, Soluble form, Suboptimal amounts, Subtype virus, Superstimulatory, Superstimulatory function, Superstimulatory influenza virus, Superstimulatory influenza viruses, Superstimulatory protein, Synergistic effects, Transduction, Transduction machinery, Transgenic mice, Triplicate determinations, Virus.
Abstract
Abstract: The influenza virus glycoprotein hemagglutinin (HA) behaves as a superstimulatory protein for B lymphocytes from various species. Polyclonal B cell stimulation mediated by HA can be blocked by soluble anti-Ig antibodies. We here report that, if presented in a highly organized form, i.e., as anti-Ig mAb coupled to dextran (anti-Ig-Dex), conventional BCR-ligands and influenza viruses act synergistically on murine B cell activation. Proliferative responses of both spellen-derived and peritoneal B cells mediated by suboptimal amounts of HA were significantly augmented by costimulation with anti-Ig-Dex, and vice versa. Similarly, anti-Ig-Dex, which on its own cannot incude Ig production in the absence of added cytokines, significantly enhanced Ig synthesis in response to superstimulatory HA. By contrast, poorly organized BCR-ligands (i.e. the same anti- Ig mAb in a soluble form) had either no, or a strong inhibitory effect on virus-triggered lymphocyte activation. Assays with various second messenger-antagonists, however, revealed clear differences in the signaling pathway employed by anti-Ig-Dex and HA, suggesting that the functional synergy between the two multimeric agents is mediated by engagement of distinct transducing elements. Taken together, these results indicate that the superstimulatory function of influenza virus HA represents a molecular strategy to mimick B cell activation by conventional, highly organized particulate-antigens.
Url:
DOI: 10.1016/S0171-2985(96)80056-8
Affiliations:
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ISTEX:A638AD5133DF017B1457D97864A9A8FBBA70D818Le document en format XML
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Mond</name></author><author><name sortKey="Cash, Evelyne" sort="Cash, Evelyne" uniqKey="Cash E" first="Evelyne" last="Cash">Evelyne Cash</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A638AD5133DF017B1457D97864A9A8FBBA70D818</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/S0171-2985(96)80056-8</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-X2L344P7-Q/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001580</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001580</idno><idno type="wicri:Area/Istex/Curation">001580</idno><idno type="wicri:Area/Istex/Checkpoint">000A29</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A29</idno><idno type="wicri:doubleKey">0171-2985:1996:Rott O:superstimulatory:influenza:virus</idno><idno type="wicri:Area/Main/Merge">001D18</idno><idno type="wicri:Area/Main/Curation">001C50</idno><idno type="wicri:Area/Main/Exploration">001C50</idno><idno type="wicri:Area/France/Extraction">000284</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Superstimulatory Influenza Virus and Highly Organized BCR-Ligands Act Synergistically on B Cell Activation</title><author><name sortKey="Rott, Ortwin" sort="Rott, Ortwin" uniqKey="Rott O" first="Ortwin" last="Rott">Ortwin Rott</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Cochin Hospital, Rene Descartes University, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Mond, James J" sort="Mond, James J" uniqKey="Mond J" first="James J." last="Mond">James J. Mond</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Uniformed Services University of the Health Services, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Cash, Evelyne" sort="Cash, Evelyne" uniqKey="Cash E" first="Evelyne" last="Cash">Evelyne Cash</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Cochin Hospital, Rene Descartes University, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>aDr. E. Cash, INSERM U.283, Pavillon Hardy A, Cochin Hospital, 27. rue du Fg. Saint-Jacques, 75674 Paris Cedex 14</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunobiology</title><title level="j" type="abbrev">IMBIO</title><idno type="ISSN">0171-2985</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">196</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="332">332</biblScope><biblScope unit="page" to="349">349</biblScope></imprint><idno type="ISSN">0171-2985</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0171-2985</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Activation</term><term>Adsorption values</term><term>Antigen organization</term><term>Cell activation</term><term>Cell activators</term><term>Cell encounter</term><term>Cell proliferation</term><term>Cell proliferative responses</term><term>Cell responses</term><term>Cell responsiveness</term><term>Cell stimulation</term><term>Cell subpopulation</term><term>Cell subpopulations</term><term>Cells cells</term><term>Cholera toxin</term><term>Clear differences</term><term>Cochin hospital</term><term>Culture medium</term><term>Culture period</term><term>Different amounts</term><term>Different concentrations</term><term>Different forms</term><term>First example</term><term>Flow cytometry</term><term>High amounts</term><term>High concentrations</term><term>High rate</term><term>Immunoglobulin</term><term>Immunoglobulin synthesis</term><term>Immunol</term><term>Independent experiments</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus glycoprotein hemagglutinin</term><term>Influenza virus hemagglutinin</term><term>Influenza viruses</term><term>Inhibitory</term><term>Inhibitory effects</term><term>Lymphocyte</term><term>Lymphocyte activation</term><term>Maximal responses</term><term>Measurable production</term><term>Molecular strategy</term><term>Mond</term><term>Murine</term><term>Neonatal mice</term><term>Obligatory role</term><term>Other hand</term><term>Pathway</term><term>Phorbol esters</term><term>Phosphodiesterase inhibitor pentoxifylline</term><term>Proliferative</term><term>Proliferative responses</term><term>Reagent</term><term>Receptor</term><term>Respective stimulus</term><term>Responder cells</term><term>Rott</term><term>Saline solution</term><term>Saturating concentrations</term><term>Side scatter parameters</term><term>Significant proliferative responses</term><term>Similar observations</term><term>Small amounts</term><term>Soluble</term><term>Soluble antibodies</term><term>Soluble form</term><term>Suboptimal amounts</term><term>Subtype virus</term><term>Superstimulatory</term><term>Superstimulatory function</term><term>Superstimulatory influenza virus</term><term>Superstimulatory influenza viruses</term><term>Superstimulatory protein</term><term>Synergistic effects</term><term>Transduction</term><term>Transduction machinery</term><term>Transgenic mice</term><term>Triplicate determinations</term><term>Virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The influenza virus glycoprotein hemagglutinin (HA) behaves as a superstimulatory protein for B lymphocytes from various species. Polyclonal B cell stimulation mediated by HA can be blocked by soluble anti-Ig antibodies. We here report that, if presented in a highly organized form, i.e., as anti-Ig mAb coupled to dextran (anti-Ig-Dex), conventional BCR-ligands and influenza viruses act synergistically on murine B cell activation. Proliferative responses of both spellen-derived and peritoneal B cells mediated by suboptimal amounts of HA were significantly augmented by costimulation with anti-Ig-Dex, and vice versa. Similarly, anti-Ig-Dex, which on its own cannot incude Ig production in the absence of added cytokines, significantly enhanced Ig synthesis in response to superstimulatory HA. By contrast, poorly organized BCR-ligands (i.e. the same anti- Ig mAb in a soluble form) had either no, or a strong inhibitory effect on virus-triggered lymphocyte activation. Assays with various second messenger-antagonists, however, revealed clear differences in the signaling pathway employed by anti-Ig-Dex and HA, suggesting that the functional synergy between the two multimeric agents is mediated by engagement of distinct transducing elements. Taken together, these results indicate that the superstimulatory function of influenza virus HA represents a molecular strategy to mimick B cell activation by conventional, highly organized particulate-antigens.</div></front></TEI><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Maryland</li><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Rott, Ortwin" sort="Rott, Ortwin" uniqKey="Rott O" first="Ortwin" last="Rott">Ortwin Rott</name></region><name sortKey="Cash, Evelyne" sort="Cash, Evelyne" uniqKey="Cash E" first="Evelyne" last="Cash">Evelyne Cash</name><name sortKey="Cash, Evelyne" sort="Cash, Evelyne" uniqKey="Cash E" first="Evelyne" last="Cash">Evelyne Cash</name></country><country name="États-Unis"><region name="Maryland"><name sortKey="Mond, James J" sort="Mond, James J" uniqKey="Mond J" first="James J." last="Mond">James J. 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